Nitrogen Excretion In The Urine

Research conducted by experts on the mechanisms of nitrogen fixation reaction using only a pimple roots and bacterial nitrogen catcher. The results of their research is that the nitrogen captured from the air by the bodies had been converted into ammonia. This means that in the body is held hydrogenation or reduction system. Not only N - N are reduced to NH 3 but other compounds such as acetylene, cyanide, and azide can also be reduced by fastening the body of nitrogen. Therefore, in the process of hydrogen or electrons needed to reduce it in such a system must have a compound that acts as hydrogen or electron donor.

Nitrification is a process of oxidation of ammonia to nitrite and subsequently into nitrate. The conversion was carried out by soil bacteria, micro-organism from which it acquires the energy for life. The reaction that takes place in stages, due oxidation of ammonia to nitrite is carried out by aerobic bacteria Khemolitotrop, nitrosomonas. Electrons derived from ammonia flows to the oxygen through sitokhrom. Enzyme that catalyzes the electron transport is to form a single system called catalyzes electron transport is to form a single system called hidrogenas system. In this electron transport phosphorylation process occurs.

In the same way nitrite is the result of changes in ammonia by Nitrosomonas waste, used by Nitrobacter. These bacteria convert nitrite to nitrate. Changes in ammonia to nitrite and then nitrate help supply N to crops. Nitrates are absorbed by the plants gradually converted into ammonia which in turn is used in the process aminasi.

Method of nitrogen secretion in each organism is different from one another. Primates excrete uric acid as the end result of purine metabolism, whereas in other mammals first became alantoin mendegradasikannya before excreted. In most species are able to degrade even further to form gliosilik acid and urea and excreted through urine. Nitrogen excretion is closely related to the nutritional status of living things. Decreased levels of N in the diet will cause a significant decrease in N excretion in the urine.

Protein compounds can be obtained from food. Proteins of animal origin referred to animal protein, while those originating from animals known as vegetable protein. Plants forming protein from CO2, H2O and nitrogen compounds. Animals that eat plants convert plant protein into animal protein. Besides being available for the formation of the body's cells, proteins can also be used as a source of energy when our body carbohydrate and fat deficiency. The average composition of the elements contained in the protein is as follows: Carbon 50%, hydrogen 7%, Oxygen 23%, Nitrogen 16%, 0-3% sulfur, and phosphorus 0-3%.

By referring to the nitrogen content of 16%, protein content determination can be done in a food ingredient. The element nitrogen is determined quantitatively, for example by Kjeldahl method, that is by destruction with concentrated acid. Weight protein that is determined is 6.25 times the weight of nitrogen.

Raw foods consist of a compound was intact complex in the small intestine eventually converted into simple molecules are easily absorbed through the intestinal wall into the blood or spleen are at particular classes of compounds absorbing lipids. Time of food that can not be digested channeled through the urine channel and the large intestine to release. In animal excretion of nitrogen in the form of urine.

To analyze the nitrogen content in urine by Kjeldahl method, which through 3 Stage: Sample preparation, digestion, distillation.

Stage Preparation

Samples were performed by including 3 grams of catalyst (CU2SO4: K2SO4 = 1: 2) 4 ml H2SO4 1 ml of urine into the Kjeldahl flask and the obtained results form a black solution.

Stage Destruction

This phase is aimed at solving the protein with the aid of concentrated sulfuric acid and by using catalyst Cu2SO4: K2SO4. In the destruction phase of the samples that have been made and heated until complete destruction phase solution previously obtained results in black turned into a clear blue color.

Pumpkin was the result of preparation put in the heater with a small fire, after the solution began to black, pumpkin rotated slowly until the color clear. After the clear color to survive 1 hour, destruction stopped. From the black solution after didestruksi turned into a clear blue color.

Stage distillation

Distilled mixture of nitrogen and ammonia are released and captured by the borax acid and the resulting distillate is blue and then titrated with HCl 0.2 N as 0.6 ml until the solution changes color to purple. The color purple prove the existence of nitrogen in the urine.

In this distillation stage of the destruction was diluted to 300 ml volume. Preparing Erlenmayer 650 ml filled with 50 ml 100 ml 0.1 N H3BO3 aquades mix 3 drops of indicator. Kjehldal pumpkin container and placed in the distillation equipment. Coolant flow and keep the coolant temperature is not more than 70o F. In the pumpkin kjehldal included Zn metal and 75 ml ¬ ¬ ¬ 50 ml NaOH (through walls). Heating a small fire lit on Distillation was stopped after the volume reached 300 ml. Results distillation titrated with 0.2 N HCL to change color.