Friday, October 8, 2010

Testing Of Wheat Flour And The Color Reaction

The way the work done on the testing of wheat flour and the color reaction is:
The first is test of wheat flour have 2 experiment.

Fat stain test. Testing menggojok fat with a little flour with the ester, and then test the air-ether solution of the fat with osmiat acid and observed results.


Protein Test. Testing of protein by mixing flour and water until it becomes dough, and then placed over the fabric, folded and massage in a place containing water, are repeated by using new water, after which the washing water stored earlier. Further testing the clot that remains in the cloth to the protein by observing the color reaction.

Both the color reaction with Biuret test, Millon test, test tryptophan (Hopkins-cole), Xantho test Protein, and Starch test.

Biuret Test. Taking 2 samples in a test tube that poured 2 ml 10% KOH (or 1 ml 40% NaOH), then with the correct way of adding them and observing the color reaction.

Millon test. Take 1 ml of protein solution added to 1 ml merkurisulfat solution (1% HgSO4 poured into 10% sulfuric acid, was observed in comparison). Then immediately heated to boiling and the observed changes, after it cooled under running water and then added drop by drop 1% NaNO3 solution, heated again and observed the changes that occur.

Tryptophan test (Hopkins-cole). Pour 1 ml protein solution in a test tube and 1 ml of diluted formaldehyde solution, then added 1 ml of concentrated H2SO4 through the tube wall resulting in two layers, and observed the changes that followed digojok.
Xantho test protein. Filling a test tube with 3 ml of protein solution and 1 ml concentrated HNO3, then boiled, and cooled quickly by dividing the contents into two tubes. After that a single tube is poured with ammonia and observed by comparing the changes that occur in the second tube.

Starch Test. Pour a drop of water washing over the glass object, and then viewed under a microscope and the starch granules to be rendered. Furthermore, using starch solution to test Yod and observed the color reaction, then heated and noted the color changes.

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